@article {doi:10.1161/CIRCRESAHA.123.322588,
	title = {Stretch Harmonizes Sarcomere Strain Across the Cardiomyocyte},
	journal = {Circulation Research},
	year = {2023},
	month = {07/2023},
	publisher = {American Heart Association},
	abstract = {BACKGROUND:Increasing cardiomyocyte contraction during myocardial stretch serves as the basis for the Frank-Starling mechanism in the heart. However, it remains unclear how this phenomenon occurs regionally within cardiomyocytes, at the level of individual sarcomeres. We investigated sarcomere contractile synchrony and how intersarcomere dynamics contribute to increasing contractility during cell lengthening.METHODS:Sarcomere strain and Ca2+\&nbsp;were simultaneously recorded in isolated left ventricular cardiomyocytes during 1 Hz field stimulation at 37\&thinsp;\&deg;C, at resting length and following stepwise stretch.RESULTS:We observed that in unstretched rat cardiomyocytes, differential sarcomere deformation occurred during each beat. Specifically, while most sarcomeres shortened during the stimulus, \&asymp;10\% to 20\% of sarcomeres were stretched or remained stationary. This nonuniform strain was not traced to regional Ca2+\&nbsp;disparities but rather shorter resting lengths and lower force production in systolically stretched sarcomeres. Lengthening of the cell recruited additional shortening sarcomeres, which increased contractile efficiency as less negative, wasted work was performed by stretched sarcomeres. Given the known role of titin in setting sarcomere dimensions, we next hypothesized that modulating titin expression would alter intersarcomere dynamics. Indeed, in cardiomyocytes from mice with titin haploinsufficiency, we observed greater variability in resting sarcomere length, lower recruitment of shortening sarcomeres, and impaired work performance during cell lengthening.CONCLUSIONS:Graded sarcomere recruitment directs cardiomyocyte work performance, and harmonization of sarcomere strain increases contractility during cell stretch. By setting sarcomere dimensions, titin controls sarcomere recruitment, and its lowered expression in haploinsufficiency mutations impairs cardiomyocyte contractility.},
	url = {https://www.ahajournals.org/doi/abs/10.1161/CIRCRESAHA.123.322588},
	author = {Li, Jia and Sundnes, Joakim and Hou, Yufeng and Laasmaa, Martin and Ruud, Marianne and Unger, Andreas and Terje R. {Kolstad} and Frisk, Michael and Per A. {Norseng} and Yang, Limin and Ingunn E. {Setterberg} and Estela S. {Alves} and Kalakoutis, Michaeljohn and Ole M. {Sejersted} and Johanna T. {Lanner} and Wolfgang A. {Linke} and Ida G. {Lunde} and Pieter P. {de Tombe} and William E. {Louch}}
}
